(Rac)-Idroxioleic acid (25-75 μM; 72 hours) impairs Jurkat cell growth in a time- and concentration-dependent manner, with an IC50 of ~40 μM.
(Rac)-Idroxioleic acid (25-50 μM; 72 hours) also induces a marked and concentration-dependent increase in the proteolytic cleavage of PARP, a molecular marker of apoptosis.
Cell Viability Assay
Cell Line: |
Jurkat cells |
Concentration: |
25 μM, 50 μM, 75 μM |
Incubation Time: |
24 hours, 48 hours, 72 hours |
Result: |
Impaired Jurkat cell growth in a time- and concentration-dependent manner. |
Western Blot Analysis
Cell Line: |
Jurkat cells |
Concentration: |
25 μM, 50 μM |
Incubation Time: |
72 hours |
Result: |
Induced a marked and concentration-dependent increase in the proteolytic cleavage of PARP. |