hnRNPK-IN-1 (Compound 25; 1.25-5 μM; 24 hours) treatment induces Hela cells apoptosis could be due to its repression of cmyc transcription.
hnRNPK-IN-1 (1.25-5 μM; 48 hours) treatment down-regulates c-myc gene transcription and expression in Hela cells in a dose-dependent manner.
hnRNPK-IN-1 shows a selective anti-proliferative effect on human cancer cell lines (Siha, A549, Hela, U2OS, A375, HuH7 and HEK293 cells) with IC50 values ranged from 1.36 to 3.59 μM.
hnRNPK-IN-1 could selectively bind to hnRNP K without significant interaction with c-myc promoter DNA. The binding of hnRNPK-IN-1 to hnRNP K could disrupt the interaction between hnRNP K and c-myc promoter DNA in vitro and in cells.
Apoptosis Analysis
Cell Line: |
Hela cells
|
Concentration: |
1.25 μM, 2.5 μM, 5 μM |
Incubation Time: |
24 hours |
Result: |
Induced Hela cells apoptosis and apoptosis-related protein expressions. |
RT-PCR
Cell Line: |
Hela cells
|
Concentration: |
1.25 μM, 2.5 μM, 5 μM |
Incubation Time: |
48 hours |
Result: |
Caused a reduction of c-myc mRNA in a dose-dependent manner in Hela cells. |
Western Blot Analysis
Cell Line: |
Hela cells
|
Concentration: |
1.25 μM, 2.5 μM, 5 μM |
Incubation Time: |
48 hours |
Result: |
The expression levels of c-MYC also decreased in a dose-dependent manner. |