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Eact is a selective and potent activator of TMEM16A, directly activates the TRPV1 channels in sensory nociceptors and produces itch, acute nociception and thermal hypersensitivity.

Eact elicits both itch-and pain- related behaviours in a TRPV1-dependent manner. Eact activates membrane currents in mTRPV1-expressing HEK293T cells in a concentration-dependent manner with an EC₅₀ of 11.6 ± 2.5 μM. TMEM16A activator Eact activates both native and recombinant TRPV1 channels.

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