3-Methyladenine - CAS:5142-23-4

规格	价格	是否有货
50mg		In-stock
100mg		In-stock
200mg		In-stock
500mg		In-stock
1g	询价	In-stock
5g	询价	In-stock

规格

价格

是否有货

数量

50mg

In-stock

100mg

In-stock

200mg

In-stock

500mg

In-stock

询价

In-stock

询价

In-stock

Other Forms of Rapamycin:

生物活性

3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K.

体外研究

3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner.
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions.
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy.
3-Methyladenine induces cell death is independent of autophagy inhibition.
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest.
Note:
在体外细胞培养实验中，3-MA 常用的浓度为 0.5-10 mM，所以优先推荐直接称取本次实验所需粉末量，用培养基完全溶解，0.22 μm 过滤器过滤除菌之后使用。建议溶液现配现用，尽快用完。

Cell Viability Assay

Cell Line:	HeLa cells
Concentration:	0 mM, 2.5 mM or 5 mM, 10 mM
Incubation Time:	0 hour, 24 hours and 48 hours
Result:	Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation.

Cell Autophagy Assay

Cell Line:	HeLa cells
Concentration:	5 mM
Incubation Time:	24 hours
Result:	Suppressed autophagy in HeLa cells under both glucose-free conditions and normal conditions.

Western Blot Analysis

Cell Line:	HeLa cells
Concentration:	5 mM
Incubation Time:	0 hour, 12 hours, 24 hours and 48 hours
Result:	Suppressed conversion of LC3-I to LC3-II between 12 hours and 48 hours.

体内研究

3-Methyladenine (1.5 mg/100 g; intraperitoneal injection; 3-24 hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24 hours.
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP.
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP.

Animal Model:	10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290 g)
Dosage:	1.5 mg/100 g (1000 μM)
Administration:	Intraperitoneal injection
Result:	Alleviated Sodium Taurocholate-Induced SAP.

分子式

C₆H₇N₅

分子量

149.15

CAS号

5142-23-4

中文名称

3-甲基腺嘌呤

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

H₂O : 5 mg/mL (33.52 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	6.7047 mL	33.5233 mL	67.0466 mL
5 mM	1.3409 mL	6.7047 mL	13.4093 mL
10 mM	0.6705 mL	3.3523 mL	6.7047 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： PBS
Solubility: 4 mg/mL (26.82 mM); Clear solution; Need ultrasonic

科研文献

纯度： 98%

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产品说明书

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COA

Other Forms of Rapamycin:

KKL 顾客使用本产品发表的 290 篇科研文献

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