BD750 - CAS:892686-59-8

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BD750

目录号 : KM10727 CAS No. : 892686-59-8 纯度 : 98%

BD750 是一个有效的免疫抑制剂，是JAK3/STAT5 的抑制剂，可抑制 IL-2 诱导的、JAK3/STAT5 依赖的 T 细胞增殖，其抑制小鼠和人 T 细胞增殖的IC₅₀ 值分别为 1.5 μM 和 1.1 μM。

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10mg		In-stock
25mg		In-stock
50mg		In-stock
100mg		In-stock
200mg	询价	In-stock
500mg	询价	In-stock

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生物活性

BD750, an effective immunosuppressant and a JAK3/STAT5 inhibitor, inhibits IL-2-induced JAK3/STAT5-dependent T cell proliferation, with IC₅₀ values of 1.5 μM and 1.1 μM in mouse and human T cells, respectively.

体外研究

BD750 inhibits human T cell proliferation stimulated either by anti-CD3/anti-CD28 mAbs or by alloantigen in a dose-dependent manner with IC₅₀ values of 1.1 ± 0.2 μM and 1.3 ± 0.2 μM respectively.
BD750 also inhibits ConA, PMA/ionomycine or alloantigen-induced mouse T cell proliferation and PHA or PMA/ionomycine-induced human T cell proliferation.
BD750 (5 or 20 μM) inhibits the LPS-induced JAK-STAT5 signaling in DC.

Western Blot Analysis

Cell Line:	Purified immature DCs.
Concentration:	1, 5 or 20 μM.
Incubation Time:	12 h.
Result:	Had fewer small dendrites and smaller clusters than typical mDCs (5 or 20 μM). At a higher dose significantly reduced the levels of LPS-stimulated IL-6, IL-12, TNF-α, IL-1β and IL-23 production by DCs.

体内研究

BD750 can induce tolerogenic dendritic cells (tolDC) and their function in experimental autoimmune encephalitis (EAE) in mice.

Animal Model:	Female C57BL/6 mice (10 wks old, 19-21 g).
Dosage:	20 μM.
Administration:	IV, treated DC on d 7, 11 and 15 post the first PTX injection (dpi 7, 11 and 15).
Result:	Significantly reduced the frequency of Th1 and Th17 cells and increased the percentage of Tregs compared with mice receiving PBS.

分子式

C₁₄H₁₃N₃Os

分子量

271.34

CAS号

892686-59-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 125 mg/mL (460.68 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.6854 mL	18.4271 mL	36.8541 mL
5 mM	0.7371 mL	3.6854 mL	7.3708 mL
10 mM	0.3685 mL	1.8427 mL	3.6854 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (7.67 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.67 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

纯度： 98%

Data Sheet

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