Camptothecin - CAS:7689-03-4

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Camptothecin (Synonyms:喜树碱; Campathecin; (S-(+)-Camptothecin; CPT))

目录号 : KM10664 CAS No. : 7689-03-4 纯度 : 98%

Data Sheet 产品使用指南

Camptothecin (CPT)，一种生物碱，是一种 DNA topoisomerase I (Topo I) 抑制剂，其 IC₅₀ 值为 679 nM。Camptothecin (CPT) 对结直肠癌、乳腺癌、肺癌和卵巢癌具有强大的抗肿瘤活性，通过改变人类癌细胞中的 miRNA 表达模式来调节 hypoxia-inducible factor-1α (HIF-1α) 活性。

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生物活性

Camptothecin (CPT), a kind of alkaloid, is a DNA topoisomerase I (Topo I) inhibitor with an IC₅₀ of 679 nM. Camptothecin (CPT) exhibits powerful antineoplastic activity against colorectal, breast, lung and ovarian cancers, modulates hypoxia-inducible factor-1α (HIF-1α) activity by changing microRNAs (miRNA) expression patterns in human cancer cells.

体外研究

High TOP1 enzymatic activity MCF7 (Luminal subtype) and HCC1419 (HER2 subtype) show high sensitivity toward Camptothecin (0.1 μM to 5 μM; 72 hours) treatment, exhibiting the IC₅₀ values of 0.089 μM and 0.067 μM, respectively.
Camptothecin (0.5 μM; 6 and 24 hours) reduces desferrioxamine-activated VEGF expression.
Camptothecin (0.5 μM; 8 to 24 hours) strongly prevents the desferrioxamine-dependent HIF-1a accumulation.

Cell Viability Assay

Cell Line:	MCF7 (Luminal subtype) and HCC1419 (HER2 subtype)
Concentration:	0.1 μM to 5 μM
Incubation Time:	72 hours
Result:	High TOP1 enzymatic activity MCF7 (Luminal subtype) and HCC1419 (HER2 subtype) show high sensitivity toward CPT (0.1 μM to 5 μM; 72 hours) treatment, exhibiting the IC₅₀ values of 0.089 μM and 0.067 μM, respectively.

RT-PCR

Cell Line:	HeLa and HEK293 cell lines
Concentration:	0.5 μmol/L
Incubation Time:	6 and 24 hours
Result:	Reduces desferrioxamine-activated VEGF expression in both cell lines after 6 and 24 hours of treatment, whereas in normoxic condition camptothecin does not affect the VEGF mRNA level.

Western Blot Analysis

Cell Line:	HeLa and HEK293 cell lines
Concentration:	0.5 μmol/L
Incubation Time:	8 to 24 hours
Result:	Strongly prevents the desferrioxamine-dependent HIF-1a accumulation after 8 to 24 hours, whereas it does not affect HIF-1b levels.

体内研究

Camptothecin (2 mg/kg every other day) treats mice, has developed numerous pulmonary metastases.
Treatment with both kinase inhibitor of nuclear factor-kappaB-1 (KINK-1) and Camptothecin led to a statistically significant reduction in the number of pulmonary metastases.

Animal Model:	C57BL6 mice (injected with B16F10 melanoma cells)
Dosage:	2 mg/kg
Administration:	every other day, after 19 days
Result:	Has developed numerous pulmonary metastases.

分子式

C₂₀H₁₆N₂O₄

分子量

348.35

CAS号

7689-03-4

中文名称

喜树碱

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 6.25 mg/mL (17.94 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.8707 mL	14.3534 mL	28.7068 mL
5 mM	0.5741 mL	2.8707 mL	5.7414 mL
10 mM	0.2871 mL	1.4353 mL	2.8707 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

临床试验

科研文献

纯度： 98%

Data Sheet

产品使用指南

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量

浓度

体积

分子量*

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度

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浓度

体积

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Other Forms of Rapamycin:

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